Journal: Journal of Oral Microbiology
Article Title: Vendor-specific microbiomes influence oral cancer development and its response to Streptococcus mitis intervention in mice
doi: 10.1080/20002297.2025.2611642
Figure Lengend Snippet: Study design. Six-week-old C57BL/6 mice from Taconic and Jackson laboratories (balanced by sex) were acclimated for one week, then randomised to receive vehicle or Streptococcus mitis (administered twice weekly). All mice were exposed to 100 µg/mL 4-nitroquinoline-1-oxide (4-NQO) in drinking water for 8 weeks to induce oral carcinogenesis, and followed up for an additional 24 weeks. Oral swab and faecal samples were collected at baseline (pre-4-NQO) and at week 8 (post-4-NQO) and were subjected to microbiome analysis by 16S rRNA gene sequencing. Bacterial intervention was discontinued at week 28. At the end of the follow-up period, mice were euthanized and tongues harvested for macroscopic and histopathological analysis.
Article Snippet: S. mitis ATCC 49456 was revived overnight from frozen stocks in Brain Heart Infusion (BHI) broth at 37 °C, sub-cultured the following morning and allowed to grow to mid-logarithmic phase (OD600 ~ 0.5).
Techniques: Sequencing